Moulds and asthma: time for indoor climate change?

T he spectre of indoor moulds as a contributor to respiratory disease keeps raising its fruiting body and just won’t go away. Numerous studies support a circumstantial and temporal link between high mould exposure and worse symptoms in susceptible individuals. However, it seems that the majority of respiratory physicians (at least in Europe) are at best non-believers. They are reluctant to consider moulds as important in patients with respiratory symptoms, rarely make specific enquiry, and almost never make attempts to reduce mould exposure. This contrasts with enthusiasm bordering on evangelism from some experts in the USA where huge litigation raises the stakes, with over 10 000 cases pending and multi-million settlements already routine. In the past we have been hindered by profound ignorance of the biology of these important environmental contaminants. What do we know about indoor moulds, and how are they implicated in respiratory diseases, and specifically asthma? Should we be trying to reduce mould exposure for specific patients or the whole population and, if so, how? Evidence for outdoor mould exposure and exacerbations of asthma is strong. For example, Alternaria is the dominant allergen in the mid west USA with strong temporal relationships between exposure and asthma severity. There are huge airborne spore counts (1000 times grass pollen counts) on peak days, associated with immediate worsening in sensitised subjects and increased asthma deaths. However, assessing indoor mould exposure and relating exposure to worse respiratory disease is a much more complex issue. A range of mould species is undoubtedly associated with serious respiratory disease including infection (sinus and pulmonary) and allergy (allergic bronchopulmonary aspergillosis). In addition, there is strong evidence for a link between severe asthma and indoor mould sensitisation. But it has been unclear whether this association is an epiphenomenon reflecting worse non-specific atopy, or whether specific avoidance or treatment for fungi might be beneficial. Several confounding issues prevent an immediate conclusion: skin prick test solutions for moulds are crude, vary by manufacturer and may be nonspecific, and severe asthma could be a reflection of a severe reaction to environmental mould exposure or require colonisation of the airways (or skin). Novel and sensitive molecular methods may now help resolve this, since they identify apparently frequent fungal colonisation of sputum. This will avoid the need for culture and identification (which is highly skilled, selects fungi by their culture characteristics and may distort patterns of true exposure). A new acronym has recently been proposed for severe asthma with fungal sensitisation (SAFS); anecdotal responses to antifungal therapy have been observed, and a doubleblind randomised controlled trial of itraconazole is nearing completion. The fungal kingdom is thought to contain over a million species, of which about 80 000 have been named and about 600 species cause some form of human disease. In contrast, most infectious diseases of plants are fungal in origin. To date, very few airborne genera i.e. Alternaria, Aspergillus, Cladosporium, Botrytis and Penicillium have been implicated in allergic asthma, and a few more in extrinsic allergic alveolitis. A number of factors may make these species respiratory allergens. First, fungi such as Aspergillus and Penicillium have a spore size (,5 mm) within the respirable range, unlike other fungi which have much larger spores. Second, some fungi such as Aspergillus fumigatus which is capable of growth at 37̊ C can germinate and colonise the sinuses and airways, unlike Aspergillus clavatus, for example (the cause of malt workers’ lung), which barely survives at 37̊ C. Third, most fungal allergens fall into specific protein types, although a few key ones are unique to a species. A fumigatus is best explored and over 60 allergens are described with a wide range of biological effects. Proteases may be important in fungal antigen penetration of the airway mucosa but could also have a broader role in permitting sensitisation to other nonprotease environmental allergens (such as cat or dog proteins). Importantly, preformed allergen may be inhaled on the surface of hyphal fragments. Fourth, nonallergic mechanisms could act in parallel or independently. Glucan is a pro-inflammatory component of fungal cell walls and fungi also produce a range of volatile fungal mycotoxins, the importance of which we do not know with respect to respiratory disease. Remarkably, we still do not understand what actually constitutes airborne mould exposure and where it comes from. The public and physicians alike consider indoor mould as that black (and virtually indestructible) rind in the shower, or where there is water penetration onto walls and ceiling often in low income households. However, there may be other important sources of mould. We have recently cultured a large range of moulds in pillows, together with volatile mycotoxins detected in low concentrations by mass spectrometry. The mould flora varied with type of pillow (synthetic vs feather). With the recent almost universal trend towards synthetic quilts and pillows, direct inhalation from bedding may be an important exposure in the context of allergy or infection in, for example, immunosuppressed patients. There is even evidence to suggest that Trichophyton nail infection can exacerbate asthma, which improves with specific treatment. 14 New techniques of exposure assessment using nasal samplers and silent ion charged plates, together with multiplex technology for measuring multiple allergens simultaneously, provide hope for some future clarification of exposure. However, it is likely to be horrendously complex and, as a result, contentious. In the absence of data on individual exposure, can we make any recommendations on control measures targeted at indoor moulds and improved health outcomes? Well, dampness is bad for asthma but good for mould growth. In this issue of Thorax, Burr and colleagues have examined asthma control after reducing dampness and removing mould in a randomised controlled trial in patients in South Wales with visible indoor mould in their homes (see page 767). Patients were randomised to either a simple intervention (mould removal plus fungicide applied to visible mould plus loft fan to promote ventilation) or control. At the end of the study the intervention group had lower humidity and about half the visible mould of the controls at 12 months (40% vs 78%). Unexpectedly, there was a trend for less improvement in peak expiratory flow variability in the intervention group than in the control group, but there were significant improvements in symptoms and reduced medication use. This is an important study, but it must be interpreted with caution. There are many potential EDITORIALS 745